Effect of rhizobium isolates and nitrogen on nodulation, growth and yield in lentil

Muhammad Tarek, Rubaiyat Binte Billah, Saiyera Chowdhury

Abstract


The Experiments were setup in three locations, Magura (23°3´ N 89°2´ E), Rangpur (25°7´ N 89°3´ E) and Mymensingh (24°8´ N 90°0´ E), Bangladesh to know the effect of two bacterial strains and 3 nitrogen doses on nodulation, yield and biomass production. The experimental material consists of one lentil popular variety viz., Binamasur-5 which was inoculated with two promising rhizobial starins/isolates namely LB-21 and LB-40 and three levels of nitrogen viz., 25, 50 and 75 kg N ha-1. The inoculant treatments were un-inoculated control (I0), LB-21 (I1), LB-40 (I2) and mixture of LB-21 and LB-40 (I1+I2). Results indicated that nodule number, nodule weight, straw yield and seed yield at the three locations were greater in bacterial isolates treated plants than in control plants. Results further indicated that mixture of two bacterial isolates had no superior effect against any single bacterial isolate. The two isolates (LB-21 and LB-40) had almost similar greater effect on nodule number, nodule weight, physiological parameters and seed yield with being the highest in LB-40. Application of 25 kg N ha-1 under both bacterial isolates performed the best in seed yield. Application of nitrogen at the rate of 75 kg ha-1 had adverse effect on nodulation, growth and seed yield in lentil. Therefore, for getting higher seed yield in lentil, should apply N as basal dose at the rate of 25 kg N ha-1 and the bacterial isolate, LB-40 may be registered as biofertilizer after few more trials in the different lentil growing areas of Bangladesh.

Keywords


Bacterial isolates, Nitrogen, Nodulation, Yield, Lentil

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References


Ali, M. A., M. Hussain, W. H. Malik and M. Tahir, 2004. Evaluation of the effect of rhizobium inoculation and phosphorus, grain yield and nodulation behaviour of lens culinaris. Pakistan journal of life social science, 22: 112-114.

Beadle, C., 1985. Plant growth analysis. In: Techniques in bioproductivity and photosynthesis. Elsevier: pp: 20-25.

Corstanje, R., K. Reddy, J. Prenger, S. Newman and A. Ogram, 2007. Soil microbial eco-physiological response to nutrient enrichment in a sub-tropical wetland. Ecological indicators, 7(2): 277-289.

Dutta, R., M. Mondal and A. Podder, 1998. Response of lentil to Bradyrhizobial strains in relation to nitrate reduction and energy. Lentil experimental news service(25): 55-59.

Gan, Y., K. Hanson, R. Zentner, F. Selles and C. McDonald, 2005. Response of lentil to microbial inoculation and low rates of fertilization in the semiarid Ccanadian prairies. Canadian journal of plant science, 85(4): 847-855.

Hossain, M. E., I. F. Chowdhury, M. Hasanuzzaman, S. Mazumder, M. A. Matin and R. Jerin, 2014. Effect of nitrogen and bradyrhizobium on growth and yield of mungbean. Journal of biosciences and agriculture research, 1(02): 79-84.

Htwe, A. Z., S. M. Moh, K. M. Soe, K. Moe and T. Yamakawa, 2019. Effects of biofertilizer produced from bradyrhizobium and streptomyces griseoflavus on plant growth, nodulation, nitrogen fixation, nutrient uptake, and seed yield of mung bean, cowpea, and soybean. Agronomy, 9(2): 77.

Huang, J., R. Keshavarz Afshar and C. Chen, 2016. Lentil response to nitrogen application and rhizobia inoculation. Communications in soil science plant analysis, 47(21): 2458-2464.

Huang, J., R. Keshavarz Afshar, A. Tao and C. Chen, 2017. Efficacy of starter n fertilizer and rhizobia inoculant in dry pea (Pisum sativum Linn.) production in a semi-arid temperate environment. Soil science plant nutrition, 63(3): 248-253.

Iqbal, M. A., M. Khalid, S. M. Shahzad, M. Ahmad, N. Soleman and N. Akhtar, 2012. Integrated use of rhizobium leguminosarum, plant growth promoting rhizobacteria and enriched compost for improving growth, nodulation and yield of lentil (Lens culinaris Medik.). Chilean journal of agricultural research, 72(1): 104.

Meleta, T. and G. Abera, 2019. Effects of rhizobium inoculation and phosphorus fertilizer rates on growth, yield and yield components of chickpea (Cicer arietinum L.) at goro, bale zone, oromia regional state. International journal of applied agricultural sciences, 5(3): 62.

Mondal, M., A. Puteh, M. Malek and M. Rafii, 2013. Contribution of morpho-physiological attributes on yield in lentil. Plant omics, 7: 1503-1507.

Tiwari, A., V. Prakash, A. Ahmad and R. Singh, 2018. Effect of biofertilizers and micronutrients on nutrient uptake, growth, yield and yield attributes of lentil (Lens culinaris L.). International journal of current microbiology applied sciences, 7(02): 3269-3275.

Zakeri, H. and R. J. C. S. Bueckert, 2015. Post‐flowering biomass and nitrogen accumulation of lentil substantially contributes to pod production. Crop science, 55(1): 411-419.




DOI: https://doi.org/10.33865/wjb.006.02.0410

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