Phytochemical analysis and antibacterial properties of Calotropis procera against bacterial phytopathogens

Najma Sabzal, Saima Mehr, Haneef Ur Rehman

Abstract


Calotropis procera, is known as crown flower or giant milkweed and belongs to the family Asclepiadacea. It has been traditionally used for various medicinal purposes. In the present study, the phytochemical analysis, antioxidant property, and antimicrobial activity of C. procera were evaluated. Methanol was used as a solvent for the extract preparation using soxhlet extraction. The extracts were subjected to the analysis of the different secondary bioactive metabolites. Furthermore, the antimicrobial activities of these extracts were determined against various pathogens. The qualitative analysis of plant extracts of leaves stems, and roots showed the presence of phenolic compounds, alkaloids, carbohydrates, glycosidic, protein, phytosterols, steroid, saponin, and flavonoid compounds. The leaf extract of C. procera plants inhibited 95% 2,2-diphenyl-1-picrylhydrazyl (DDPH) activity at 0.8 mg/mL Methanol extract of C. procera showed the maximum antibacterial and antifungal activities against the tested plant pathogen of bacterial and fungal strains. This research explored the various phytochemicals, including carbohydrates, glycosides, alkaloids, steroid, saponins, amino acids, phenols, and flavonoids, present in the stem, leave, root, and flower of the indigenous C. procera plant. The study gave a systematic base for the isolation of the novel bioactive phytochemicals with the antioxidant and antibacterial activities from the Calotropis species of Balochistan

Keywords


Phytochemical analysis, antioxidant, antibacterial, Calotropis procera

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References


Bader, A., Z. Omran, A. I. Al-Asmari, V. Santoro, N. De Tommasi, M. D’Ambola, F. Dal Piaz, B. Conti, S. Bedini and M. Halwani, 2021. Systematic phytochemical screening of different organs of Calotropis procera and the ovicidal effect of their extracts to the foodstuff pest Cadra cautella. Molecules, 26(4): 905.

Baloch, A. H., I. A. Baloch, I. Ahmed and S. Ahmed, 2017. A study of poisonous plants of Balochistan, Pakistan. Pure applied biology, 6(3): 989-1001.

Bobbarala, V. and V. Vadlapudi, 2009. Abrus precatorius L. Seed extracts antimicrobial properties against clinically important bacteria. International journal of pharmtech research, 1(4): 1115-1118.

Evans, W. C., 2009. Trease and evans' pharmacognosy e-book. Elsevier health sciences.

Gyawali, R., B. Bhattarai, S. Bajracharya, S. Bhandari, P. Bhetwal, K. Bogati, S. Neupane, S. Shrestha, A. K. Shrestha and R. Joshi, 2020. Α-amylase inhibition, antioxidant activity and phytochemical analysis of Calotropis gigantea (L.) dryand. Journal of health allied sciences, 10(1): 77-81.

Jack, R. W., J. R. Tagg and B. Ray, 1995. Bacteriocins of gram-positive bacteria. Microbiological reviews, 59(2): 171-200.

Larson, E. R., C. A. Lipinski and R. Sarges, 1988. Medicinal chemistry of aldose reductase inhibitors. Medicinal research reviews, 8(2): 159-186.

Magalhães, H. I., P. M. Ferreira, E. S. Moura, M. R. Torres, A. P. Alves, O. D. Pessoa, L. V. Costa-Lotufo, M. O. Moraes and Pessoa, 2010. In vitro and in vivo antiproliferative activity of Calotropis procera stem extracts. Anais da academia brasileira de ciências, 82(2): 407-416.

Mahajan, R. and S. Badgujar, 2008. Phytochemical investigations of some laticiferous plants belonging to Khandesh region of Maharashtra. Ethnobotanical leaflets, 2008(1): 151.

Meena, A. K., A. Yadav and M. Rao, 2011. Ayurvedic uses and pharmacological activities of Calotropis procera linn. Asian journal of traditional medicines, 6(2): 45-53.

Murti, Y., B. Yogi and D. Pathak, 2010. Pharmacognostic standardization of leaves of Calotropis procera (Ait.) Br. (asclepiadaceae). International journal of ayurveda research, 1(1): 14.

Nenaah, G. J. W. J. o. M., 2013. Antimicrobial activity of calotropis procera Ait (asclepiadaceae) and isolation of four flavonoid glycosides as the active constituents. World journal of microbiology and biotechnology, 29(7): 1255-1262.

Rani, R., D. Sharma, M. Chaturvedi and J. P. Yadav, 2019. Phytochemical analysis, antibacterial and antioxidant activity of Calotropis procera and Calotropis gigantea. The natural products journal, 9(1): 47-60.

Sen, S. K. and L. Behra, 2007. Ethnomedicinal plants used in touch therapy at bargarh district of orissa. Ethnobotany, 19(1&2): 100-104.

Verma, V. N., 2014. The chemical study of calotropis. International letters of chemistry, physics and astronomy, 1: 74-90.




DOI: https://doi.org/10.33865/wjb.006.02.0405

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