Muniba Abid, M. Azmat ullah Khan, Sehrish Mushtaq, Muhammad Ajmal Rana, Sohaib Afzaal, Muhammad Saleem Haider


Pesticides driven from Plants, animals, and microbes such as (bacteria fungi, viruses, algae, nematodes and protozoa are replacing traditional chemical pesticides throughout the world. Bio pesticides reduce environmental risks and are target specific. Bio pesticide promotes sustainable agriculture development by reducing the environmental pollution. Various products have been registered and released, that play important role in the agro-market. Regulation of many insect populations in nature happen by Baculoviruses (entomopathogenic viruses). The pesticide based on baculovirus particles has been formed to control pest and their use is beneficial to reduce the risk of synthetic chemical insecticides. The present status and increase use of baculovirus based bio pesticides as replacement of chemical pesticides, its role in integrated pest management, have been discussed in this review.


Baculavirus, bio control agent, Bio pesticides, Life cycle, vector

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Ashour, M.-B., D. Ragheb, E.-S. El-Sheikh, E.-A. Gomaa, S. Kamita and B. Hammock, 2007. Biosafety of recombinant and wild type nucleopolyhedroviruses as bioinsecticides. International journal of environmental research and public health, 4(2): 111-125.

Beas-Catena, A., A. Sánchez-Mirón, F. García-Camacho, A. Contreras-Gómez and E. Molina-Grima, 2014. Baculovirus biopesticides: An overview. Journal of animal and plant sciences, 24(2): 362-373.

Bézier, A., M. Annaheim, J. Herbinière, C. Wetterwald, G. Gyapay, S. Bernard-Samain, P. Wincker, I. Roditi, M. Heller and M. Belghazi, 2009. Polydnaviruses of braconid wasps derive from an ancestral nudivirus. Science, 323(5916): 926-930.

Braunagel, S. C., R. Parr, M. Belyavskyi and M. D. Summers, 1998. Autographa californicanucleopolyhedrovirus infection results in sf9 cell cycle arrest at g2/m phase. Virology, 244(1): 195-211.

Burke, G. R., S. A. Thomas, J. H. Eum and M. R. Strand, 2013. Mutualistic polydnaviruses share essential replication gene functions with pathogenic ancestors. PLoS pathogens, 9(5): e1003348.

Chen, C.-Y., C.-Y. Lin, G.-Y. Chen and Y.-C. Hu, 2011. Baculovirus as a gene delivery vector: Recent understandings of molecular alterations in transduced cells and latest applications. Biotechnology advances, 29(6): 618-631.

Chen, Y.-R., S. Zhong, Z. Fei, Y. Hashimoto, J. Z. Xiang, S. Zhang and G. W. Blissard, 2013. The transcriptome of the baculovirus autographa californica multiple nucleopolyhedrovirus in trichoplusia ni cells. Journal of virology, 87(11): 6391-6405.

Clem, R. J., 2007. Baculoviruses and apoptosis: A diversity of genes and responses. Current drug targets, 8(10): 1069-1074.

Clem, R. J. and A. L. Passarelli, 2013. Baculoviruses: Sophisticated pathogens of insects. PLoS pathogens, 9(11): e1003729.

Cory, J. S., R. S. Hails and S. M. Sait, 1997. Baculovirus ecology. In: The baculoviruses. Springer: pp: 301-339.

Doyle, C. J., M. L. Hirst, J. S. Cory and P. F. Entwistle, 1990. Risk assessment studies: Detailed host range testing of wild-type cabbage moth, mamestra brassicae (lepidoptera: Noctuidae), nuclear polyhedrosis virus. Applied envirnmental microbiology, 56(9): 2704-2710.

Gomi, S., C. E. Zhou, W. Yih, K. Majima and S. Maeda, 1997. Deletion analysis of four of eighteen late gene expression factor gene homologues of the baculovirus, bmnpv. Virology, 230(1): 35-47.

Groner, A., 1986. Specificity and safety of baculoviruses.

Guarino, L. A. and M. D. Summers, 1987. Nucleotide sequence and temporal expression of a baculovirus regulatory gene. Journal of virology, 61(7): 2091-2099.

Hang, X., W. Dong and L. A. Guarino, 1995. The lef-3 gene of autographa californica nuclear polyhedrosis virus encodes a single-stranded DNA-binding protein. Journal of virology, 69(6): 3924-3928.

Hang, X. and L. A. Guarino, 1999. Purification of autographa californica nucleopolyhedrovirus DNA polymerase from infected insect cells. Journal of general virology, 80(9): 2519-2526.

Hauschild, R., B. Speiser and L. Tamm, 2011. Regulation according to eu directive 91/414: Data requirements and procedure compared with regulation practice in other oecd countries. In: Regulation of biological control agents. Springer: pp: 25-77.

Hayakawa, T., R. Ko, K. Okano, S.-I. Seong, C. Goto and S. Maeda,

Sequence analysis of the xestia c-nigrum granulovirus genome. Virology, 262(2): 277-297.

Heimpel, A., E. Thomas, J. Adams and L. Smith, 1973. The presence of nuclear polyhedrosis viruses of trichoplusia ni on cabbage from the market shelf. Environmental entomology, 2(1): 72-75.

Hofmann, C., V. Sandig, G. Jennings, M. Rudolph, P. Schlag and M. Strauss, 1995. Efficient gene transfer into human hepatocytes by baculovirus vectors. Proceedings of the National Academy of Sciences, 92(22): 10099-10103.

Hoover, K., M. Grove, M. Gardner, D. P. Hughes, J. McNeil and J. Slavicek, 2011. A gene for an extended phenotype. Science, 333(6048): 1401-1401.

Huang, N., W. Wu, K. Yang, A. L. Passarelli, G. F. Rohrmann and R. J. Clem, 2011. Baculovirus infection induces a DNA damage response that is required for efficient viral replication. Journal of virology, 85(23): 12547-12556.

Huh, N. E. and R. F. Weaver, 1990. Identifying the rna polymerases that synthesize specific transcripts of the autographa californica nuclear polyhedrosis virus. Journal of general virology, 71(1): 195-201.

Ikeda, M., R. Hamajima and M. Kobayashi, 2015. Baculoviruses: Diversity, evolution and manipulation of insects. Entomological science, 18(1): 1-20.

Jayachandran, B., M. Hussain and S. Asgari, 2012. Rna interference as a cellular defense mechanism against the DNA virus baculovirus. Journal of virology, 86(24): 13729-13734.

Jehle, J. A., G. Blissard, B. Bonning, J. Cory, E. Herniou, G. Rohrmann, D. Theilmann, S. Thiem and J. Vlak, 2006. On the classification and nomenclature of baculoviruses: A proposal for revision. Archives of virology, 151(7): 1257-1266.

Katsuma, S., Y. Koyano, W. Kang, R. Kokusho, S. G. Kamita and T. Shimada, 2012. The baculovirus uses a captured host phosphatase to induce enhanced locomotory activity in host caterpillars. PLoS pathogens, 8(4): e1002644.

Kool, M., C. H. Ahrens, R. W. Goldbach, G. F. Rohrmann and J. M. Vlak, 1994. Identification of genes involved in DNA replication of the autographa californica baculovirus. Proceedings of the National Academy of Sciences, 91(23): 11212-11216.

Lackner, A., K. Genta, H. Koppensteiner, I. Herbacek, K. Holzmann, S. Spiegl-Kreinecker, W. Berger and M. Grusch, 2008. A bicistronic baculovirus vector for transient and stable protein expression in mammalian cells. Analytical biochemistry, 380(1): 146-148.

Lauzon, H. A., C. J. Lucarotti, P. J. Krell, Q. Feng, A. Retnakaran and B. M. Arif, 2004. Sequence and organization of the neodiprion lecontei nucleopolyhedrovirus genome. Journal of virology, 78(13): 7023-7035.

Leisy, D. J., C. Rasmussen, H.-T. Kim and G. F. Rohrmann, 1995. The autographa californica nuclear polyhedrosis virus homologous region 1a: Identical sequences are essential for DNA replication activity and transcriptional enhancer function. Virology, 208(2): 742-752.

Maeda, S., S. L. Volrath, S. A. Harper, K. Majima, D. W. Maddox, B. D. Hammock and E. Fowler, 1991. Insecticidal effects of an insect-specific neurotoxin expressed by a recombinant baculovirus. Virology, 184(2): 777-780.

Makela, A. R. and C. Oker-Blom, 2008. The baculovirus display technology-an evolving instrument for molecular screening and drug delivery. Combinatorial chemistry & high throughput screening, 11(2): 86-98.

Martignoni, M. and P. Iwai, 1981. A catalogue of viral diseases of insects, mites and ticks. In “microbial control of pests and plant diseases 1970-1980”(hd burges, ed.). Academic Press, New York/London.

McDougal, V. V. and L. A. Guarino, 2001. DNA and atp binding activities of the baculovirus DNA helicase p143. Journal of virology, 75(15): 7206-7209.

McWilliam, A., 2007. Environmental impact of baculoviruses. FAO. R7299_FTR_anx3.

Means, J. C. and A. L. Passarelli, 2010. Viral fibroblast growth factor, matrix metalloproteases, and caspases are associated with enhancing systemic infection by baculoviruses. Proceedings of the National Academy of Sciences, 107(21): 9825-9830.

Mikhailov, V. S. and G. F. Rohrmann, 2002. Baculovirus replication factor lef-1 is a DNA primase. Journal of virology, 76(5): 2287-2297.

Morris, T. D. and L. K. Miller, 1993. Characterization of productive and non-productive acmnpv infection in selected insect cell lines. Virology, 197(1): 339-348.

Nobiron, I., I. Thompson, J. Brownlie and M. E. Collins, 2003. DNA vaccination against bovine viral diarrhoea virus induces humoral and cellular responses in cattle with evidence for protection against viral challenge. Vaccine, 21(17-18): 2082-2092.

O'Reilly, D. R. and L. K. Miller, 1989. A baculovirus blocks insect molting by producing ecdysteroid udp-glucosyl transferase. Science, 245(4922): 1110-1112.

OECD, 2002. Oecd consensus document on information used in the assessment of environmental applications involving baculoviruses. Series on harmonisation of regulatory oversight in biotechnology, 20: 90.

Okano, K., A. L. Vanarsdall and G. F. Rohrmann, 2007. A baculovirus alkaline nuclease knockout construct produces fragmented DNA and aberrant capsids. Virology, 359(1): 46-54.

Ooi, B. G., C. Rankin and L. K. Miller, 1989. Downstream sequences augment transcription from the essential initiation site of a baculovirus polyhedrin gene. Journal of molecular biology, 210(4): 721-736.

Palomares, L. A., S. Estrada-Mondaca and O. Ramírez, 2006. Principles and applications of the insect-cell-baculovirus expression vector system. Cell culture technology for pharmaceutical and cellular applications: 627-692.

Passarelli, A. L. and L. A. Guarino, 2007. Baculovirus late and very late gene regulation. Current drug targets, 8(10): 1103-1115.

Peng, K., J. W. van Lent, S. Boeren, M. Fang, D. A. Theilmann, M. A. Erlandson, J. M. Vlak and M. M. van Oers, 2012. Characterization of novel components of the baculovirus per os infectivity factor complex. Journal of virology, 86(9): 4981-4988.

Possee, R. D., C. M. Griffiths, R. B. Hitchman, A. Chambers, F. Murguia-Meca, J. Danquah, A. Jeshtadi and L. A. King, 2010. Baculoviruses: Biology, replication and. Insect virology: 35.

Rapp, J. C., J. A. Wilson and L. K. Miller, 1998. Nineteen baculovirus open reading frames, including lef-12, support late gene expression. Journal of virology, 72(12): 10197-10206.

Rohrmann, G., 2008. Baculovirus molecular biology. Bethesda (md): National library of medicine (us). National Center for Biotechnology Information: 1-154.

Rohrmann, G., 2008. Baculovirus molecular biology. Bethesda (md): National library of medicine (us). National Center for Biotechnology Information.

Romanowsky, V. and P. D. Ghiringhelli, 2001. Baculovirus molecular biology: (replication and regulation of gene expression). In: Baculoviruses and their applications as bioinsecticides in the biological control of pests. Publications Service: pp: 119-142.

Serrano, A., T. Williams, O. Simón, M. López-Ferber, P. Caballero and D. Muñoz, 2013. Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants. Applied environmental microbiology., 79(4): 1118-1125.

Singh, C., J. Singh and J. Nagaraju, 2012. A baculovirus-encoded microrna (mirna) suppresses its host mirna biogenesis by regulating the exportin-5 cofactor ran. Journal of virology, 86(15): 7867-7879.

Singh, J., C. Singh, A. Bhavani and J. Nagaraju, 2010. Discovering micrornas from bombyx mori nucleopolyhedrosis virus. Virology, 407(1): 120-128.

Slack, J. and B. M. Arif, 2006. The baculoviruses occlusion‐derived virus: Virion structure and function. Advances in virus research, 69: 99-165.

Szewczyk, B., L. Hoyos-Carvajal, M. Paluszek, I. Skrzecz and M. L. De Souza, 2006. Baculoviruses—re-emerging biopesticides. Biotechnology advances, 24(2): 143-160.

Szewczyk, B., L. Rabalski, E. Krol, W. Sihler and M. L. de Souza, 2009. Baculovirus biopesticides-safe alternative to chemical protection of plants. Embrapa Genetic Resources and Biotechnology -Article in Indexed Journal (ALICE).

Thézé, J., A. Bézier, G. Periquet, J.-M. Drezen and E. A. Herniou, 2011. Paleozoic origin of insect large dsdna viruses. Proceedings of the National Academy of Sciences, 108(38): 15931-15935.

Thiem, S. M. and X.-W. Cheng, 2009. Baculovirus host-range. Virologica Sinica, 24(5): 436.

Thiem, S. M. and L. K. Miller, 1990. Differential gene expression mediated by late, very late and hybrid baculovirus promoters. Gene, 91(1): 87-94.

Volkman, L., 2007. Baculovirus infectivity and the actin cytoskeleton. Current drug targets, 8(10): 1075-1083.

Wang, R., F. Deng, D. Hou, Y. Zhao, L. Guo, H. Wang and Z. Hu, 2010. Proteomics of the autographa californica nucleopolyhedrovirus budded virions. Journal of virology, 84(14): 7233-7242.

Yang, S. and L. K. Miller, 1999. Activation of baculovirus very late promoters by interaction with very late factor 1. Journal of virology, 73(4): 3404-3409.

Zimmer, C., 2014. Mindsuckers: Meet nature's nightmare. National Geographie, 226(5).


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DOI: https://doi.org/10.33865/wjb.004.03.0221


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